Journal: bioRxiv
Article Title: Immune cells adapt to distinct stem cell niches to govern tissue homeostasis
doi: 10.64898/2026.01.28.701831
Figure Lengend Snippet: a , GeneOntlogy (GO) terms up- and down-regulated in uHF-SC upon in vivo intradermal administration of LTBR-agonist compared to isotype control injected mice. b , Normalized quantification of number of CD4 + and CD8 + T cells migrated through a transwell membrane towards the supernatant collected from uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from four independent experiments. c , Normalized quantification of number of CD8 + T cells migrated through a transwell membrane towards the supernatant collected from Bulge-SC, IFE-SC or uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from three independent experiments. d , Left: Representative max projections of whole mount immunofluorescence of the bulge, uHF and IFE compartment of wild type mice showing distribution of CD4 + cells (in white). Bulge cells are marked by Krt24 in green and upper hair follicle cells are marked by LRIG1 in light blue. Right: Quantification of the average distance of CD4 + and CD8 + T cells from Krt24 and LRIG1 quantified upon surface rendering, n =2 mice. e , qPCR analysis of Cxcl10 and Cxcl16 expression Bulge-SC, uHF-SC and IFE-SC FACS-sorted from wild type mice. Each dot represents one mouse from two independent experiments. f , Representative images of MSA plates showing bacterial colonies grown after swabbing the back skin of Ltb-CreER x R26-DTA and R26-DTA (ctrl) mice associated with S. epidermidis . Data in b and d are analysed by unpaired two-tailed Student’s t- test. Data in c and e are analysed by ordinary one-way ANOVA with Tukey’s multiple comparison post-test. p -values are indicated in each figure and data are represented as mean with SEM.
Article Snippet: When indicated, 50μg/ml of InVivoMab anti-mouse CXCL10 (IP-10, BioXcell) was added to the lower chamber.
Techniques: In Vivo, Control, Injection, Membrane, Immunofluorescence, Expressing, Two Tailed Test, Comparison